CD49b (ITGA2) Mouse Monoclonal Antibody [Clone ID: AK7]

Specifications

Product Data
Clone Name	AK7
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/50-1/100 diluted antibody to label 10e6 cells in 100 µl. Immunohistochemistry on Frozen Sections: 1/500-1/1000. Recommended Positive Control Tissue: Tonsil.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Spleen cells from immunised mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	This antibody recognises the integrin alpha 2 subunit, which is 160kD cell surface antigen expressed by NK cells and a small subset of T-cells. This antibody inhibits cell attachment to collagen.
Formulation	PBS containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha 2
Database Link	Entrez Gene 3673 Human UniProt ID P17301
Background	Integrins are heterodimeric cell surface receptors composed of alpha and beta subunits, which mediate cell-cell and cell-extracellular matrix attachments. They are responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Aberrant integrin expression has been found in many epithelial tumours. Changes in integrin expression have been shown to be important for the growth and early metastatic capacity of melanoma cells.
Synonyms	Integrin alpha-2, ITGA-2, VLA-2 alpha, VLA2, GPIa, Collagen Receptor
Reference Data

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