CD33 Mouse Monoclonal Antibody [Clone ID: WM53]

Specifications

Product Data
Clone Name	WM53
Applications	FC, IHC, IP, WB
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/10-1/50 diluted antibody to label 10e6 cells. Western blot. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/50-1/100 (Overnight incubation recommended). The epitope recognized by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommends the use of acetone fixation for frozen sections. Positive Control Tissue: Lymphatic tissue. Not suitable on Paraffin Embedded Sections.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Human AML cells. Spleen cells from immunised BALB/c mice were fused with cells of the mouse NS1 myeloma cell line.
Specificity	Recognizes the CD33 cell surface glycoprotein, a 67kD molecule expressed by monocytes, granulocyte precursors, myeloid progenitor cells and myeloid leukaemias.
Formulation	PBS, pH 7.4 State: Purified State: Liquid purified IgG fraction from Tissue Culture Supernatant Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD33 molecule
Database Link	Entrez Gene 945 Human UniProt ID P20138
Background	CD33 is found on granulocyte and macrophage precursors in the bone marrow, but is not on pluripotent stem cells. The protein is also expressed on, and is a useful marker for, peripheral monocytes. It is also useful for distinguishing myelogenous leukaemia cells from lymphoid or erythroid leukaemias.
Synonyms	Siglec-3, gp67
Reference Data

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