CD11b (ITGAM) Mouse Monoclonal Antibody [Clone ID: ICRF44]

Specifications

Product Data
Clone Name	ICRF44
Applications	FC, FN, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/10-1/100 diluted antibody to label 10e6 cells or 100 µl whole blood. Immunoprecipitation. Immunohistochemistry on Frozen Sections: 1/100-1/1000. The epitope recognised by this antibody is reported to be sensitive to formaldehyde fixation and tissue processing. We recommend the use of acetone fixation for frozen sections. Recommended Positive Control: Human tonsil.
Reactivity	Human, Monkey
Host	Mouse
Clonality	Monoclonal
Immunogen	Rheumatoid synovial cells and Human mocytes. Spleen cells from immunised BALB/c mice were fused with cells of the mouse Sp2/0 myeloma cell line.
Specificity	This antibody recognizes the CD11b cell surface glycoprotein. This antibody, clone ICRF44 has been reported to have various functional effects on monocytes, blocking adhesion and stimulating cytokine and chemokine release. Negative Species: Cat.
Formulation	PBS State: Purified State: Liquid purified IgG fraction Preservative: 0.09% Sodium Azide
Concentration	lot specific
Purification	Affinity Chromatography on Protein G
Conjugation	Unconjugated
Storage Condition	Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	integrin subunit alpha M
Database Link	Entrez Gene 3684 Human UniProt ID P11215
Background	CD11b is a 165kD molecule also known as the alphaM integrin, MAC-1 and CR3. This molecule is expressed as a heterodimer in association with the beta 2 integrin, and is found upon monocytes, granulocytes, NK cells and some peripheral blood lymphocytes.
Synonyms	ITGAM, CR3A, CR-3 alpha chain, Integrin alpha-M, MAC1
Reference Data

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