Human Lambda Light Chain Mouse Monoclonal Antibody [Clone ID: Mc24-IC6]

Specifications

Product Data
Clone Name	Mc24-IC6
Applications	ELISA, FC
Recommend Dilution	Flow cytometry: 1/10 - 1/20; use 10 µl of the suggested working dilution to label 10e6 cells or 100µl washed whole blood. ELISA
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified human IgG.
Specificity	This antibody recognises human lambda light chains, and shows no cross- reactivity to kappa light chains or to immunoglobulin heavy chains.
Formulation	PBS buffer pH 7.4 with 0.09% sodium azide as preservative State: Purified State: Liquid immunoglobulin fraction
Concentration	lot specific
Purification	Protein A affinity purified
Conjugation	Unconjugated
Storage Condition	Store the antibody at 4-8°C for one month or at -20°C for longer. This product should be stored undiluted. This product is photosensitive and should be protected from the light. Avoid repeated freezing and thawing.
Background	All five immunoglobulin classes share the same basic four polypeptide chain structure of two heavy-chains and two light chains. There are five heavy chain types, and two light-chain types (Kappa and Lambda) both having a molecular weight of 22.5kDa. Any heavy-chain type can associate with either light-chain type, but on any immunoglobulin molecule both light-chains are of the same type. Kappa and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of Kappa to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin. In normal individuals low levels of free light-chain arepresent in serum (kappa, 1.6-15.2 mg/L; Lambda, 0.4-4.2mg/L), with the occurrence of multiple myeloma or other B-cell malignancies these levels can be greatly elevated and can be found at high levels in the urine (Bence-Jones proteins).
Reference Data

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