MHC Class II RT1B (monomorphic) Mouse Monoclonal Antibody [Clone ID: OX-6]

Specifications

Product Data
Clone Name	OX-6
Applications	FC, IHC
Recommend Dilution	Flow cytometry (1:50 - 1:100): use 10 µl of the suggested working dilution to label 10e6 cells in 100 µl. This product is routinely tested in flow cytometry on rat splenocytes. Immunohistochemistry on frozen tissues and paraffin embedded sections (1:50 - 1:100): this clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde) (4). This product does not require antigen retrieval using heat treatment or protein digestion prior to staining but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6. Western blot.
Reactivity	Rat
Host	Mouse
Clonality	Monoclonal
Immunogen	Rat thymocyte membrane glycoproteins
Specificity	This antibody recognises a monomorphic determinant of the rat I-A antigen present on B lymphocytes, dendritic cells, some macrophages and certain epithelial cells. It cross reacts with certain mouse strains of MHC haplotypes k and s. Analysis of recombinant mouse strains showed that the determinants mapped to the I-A region (1). The clone does not react with Rat BDIX strain.
Formulation	PBS, pH 7.2, containing 0.09% sodium azide State: Purified State: Liquid IgG fraction
Concentration	lot specific
Purification	Protein G affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Synonyms	HLA Class II, MHC Class 2 RT1B
Reference Data

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