MHC Class II I-Ab Mouse Monoclonal Antibody [Clone ID: 25-5-16S]

CAT#: SM080FS

MHC Class II I-Ab mouse monoclonal antibody, clone 25-5-16S, FITC



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CNY 3,710.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Clone Name 25-5-16S
Applications FC
Recommend Dilution Flow Cytometry (See Protocols).
Reactivity Mouse
Host Mouse
Clonality Monoclonal
Specificity Recognizes Mouse MHC class II I-Ab, private for I-Ab.
This Monoclonal antibody specifically reacts with the I-Ab
encoded MHC class II antigen expressed on Mouse strains of the H-2b haplotype.
Formulation PBS containing 0.02% Sodium Azide as a preservative as a and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/ml
Label: FITC
State: Liquid purified IgM fraction
Concentration lot specific
Conjugation FITC
Storage Condition Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
This product is photosensitive and should be protected from light.
Avoid repeated freezing and thawing.
Background Class II antigens are most highly expressed on antigen-presenting cells including B cells, macrophages, dendritic cells and certain epithelial cells.
Synonyms H2-Eb1, H-2 class II histocompatibility antigen I-A beta chain
Note Protocol: FLOW CYTOMETRY ANALYSIS:
Method:
1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell population with Lympholyte®-M cell separation medium.
2. Wash 2 times.
3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of this suspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test).
4. To each tube, add~1.0 µg of SM080FS per 10e6 cells.
5. Vortex the tubes to ensure thorough mixing of antibody and cells.
6. Incubate the tubes for 30 minutes at 4°C.
(It is recommended that the tubes are protected from light, since most fluorochromes are light sensitive).
7. Wash 2 times at 4°C.
8. Resuspend the cell pellet in 50 µl ice cold media B.
9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidium iodide at 0.5 mg/ml in PBS. This stains dead cells by intercalating in DNA.

Media:
A. Phosphate buffered saline (pH 7.2) + 5% normal serum of host species + sodium azide (100 ml of 2M sodium azide in 100 mls).
B. Phosphate buffered saline (pH 7.2) + 0.5% Bovine serum albumin + sodium azide (100 ml of 2M sodium azide in 100 mls).

Results:
Tissue Distribution by Flow Cytometry Analysis:
Mouse strain: C57BL/6
Cell Concentration: 1x10e6 cells per test.
Antibody Concentration Used: 1.0 µg/10e6 cells.
Isotypic Control: FITC Mouse IgM
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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