Cd19 Rat Monoclonal Antibody [Clone ID: 6D5]

Specifications

Product Data
Clone Name	6D5
Applications	FC, IHC, IP
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/10-1/50 diluted antibody to label 10e6 cells in 100 µl. Immonohistochemistry on Frozen Sections. Immunoprecipitation.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Human K562 cell line transfected with murine CD19. Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
Specificity	This antibody recognises the CD19 cell surface antigen, a 95kD glycoprotein expressed by B lymphocytes. Clone 6D5 has been shown to recognise the same epitope as clone ID3 in cross-competition assays.
Formulation	PBS, pH 7.4 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD19 antigen
Database Link	Entrez Gene 12478 Mouse UniProt ID P25918
Background	CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 molecule is expressed on 100% of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10% of normal human peripheral blood cells and approximately 60% of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12% of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.
Synonyms	Leu-12, B-cell marker
Reference Data

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