Cd3e Rat Monoclonal Antibody [Clone ID: KT3]

Specifications

Product Data
Clone Name	KT3
Applications	FC, IHC
Recommend Dilution	Flow Cytometry: Use 10 µl of 1/100 diluted antibody to label 1x10e6 cells. For optimal staining incubations should be performed at room temperature. Immunohistochemistry on Frozen Sections. For optimal staining incubations should be performed at room temperature.
Reactivity	Mouse
Host	Rat
Clonality	Monoclonal
Immunogen	Spleen cells from immunised SD rats were fused with cells of the NSO mouse myeloma cell line.
Specificity	This antibody recognizes the Mouse CD3 antigen, which is expressed by mature T cells. The antibody may be used to trigger proliferation and cytotoxicity of CD3 positive cells.
Formulation	PBS, pH7.4 containing 0.09% Sodium Azide as preservative. State: Purified State: Liquid purified IgG fraction.
Concentration	lot specific
Purification	Affinity Chromatography on Protein G.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	CD3 antigen, epsilon polypeptide
Database Link	Entrez Gene 12501 Mouse UniProt ID P22646
Background	Cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Synonyms	T3/Leu-4
Reference Data

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