PARC (CUL9) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	This antibody reacts with human and mouse PARC by Western blot (1:500-1:1,000) and Immunoprecipitation. The antibody immunoprecipitates protein from cell lysates (using HeLa, NIH-3T3, and others). To date co-immuno-precipitation using 35SIP has been negative. A 281.2 kDa band corresponding to human PARC is detected. Most cell lines expressing PARC can be used as a positive control.
Reactivity	Human, Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 2503-2517 of Human PARC (C-terminus) coupled to KLH.
Specificity	This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse PARC. Cross reactivity with PARC from other sources is not known.
Formulation	State: Serum State: Liquid (sterile filtered) with 0.01% (w/v) Sodium Azide as preservative.
Concentration	lot specific
Purification	Delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Gene Name	cullin 9
Database Link	Entrez Gene 23113 Human UniProt ID Q8IWT3
Background	Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. PARC is a cullin family member that functions as a cytoplasmic anchor protein in p53-associated protein complexes. PARC regulates the subcellular localization of p53 and subsequent function. PARC forms a complex with p53 in the cytoplasm of unstressed cells and interacts with UBCH7 and UBCH8. PARC shows a cytoplasmic localization and is ubiquitously expressed in all tissues with highest expression in testis brain and kidney.
Synonyms	CUL-9, H7AP1, KIAA0708, PARC
Reference Data

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