CDC20 (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: This antibody reacts with human CDC20 (fizzy) (1:500 - 1:1,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been determined. A 54.7 kDa band corresponding to human CDC20 (fizzy) is detected. Most cell lines or tissues expressing CDC20 can be used as a positive control. ELISA: 1:2,000-1:10,000 Dilution.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 486-499 of Human CDC20 (fizzy) (C-terminal) coupled to KLH.
Specificity	This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human CDC20 (fizzy). Cross reactivity may also occur with CDC20 from other sources.
Formulation	State: Serum State: Liquid (sterile filtered) with 0.01% (w/v) Sodium Azide as preservative.
Concentration	lot specific
Purification	Delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at -20°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Gene Name	cell division cycle 20
Database Link	Entrez Gene 991 Human UniProt ID Q12834
Background	CDC20, also known as fizzy, Cell division cycle protein 20 homolog, and p55CDC is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. CDC20 is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The phosphorylated form of CDC20 interacts with APC/C. Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
Synonyms	p55CDC
Reference Data

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