CDC20 (C-term) Rabbit Polyclonal Antibody

CAT#: R1508

CDC20 (C-term) rabbit polyclonal antibody, Serum



Need it in bulk or conjugated?
Get a free quote

CNY 8,025.00


货期*
4周

规格
    • 100 ul

Product images

Specifications

Product Data
Applications ELISA, IP, WB
Recommend Dilution Western blot: This antibody reacts with human CDC20 (fizzy) (1:500 - 1:1,000).
Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins has not been determined. A 54.7 kDa band corresponding to human CDC20 (fizzy) is detected. Most cell lines or tissues expressing CDC20 can be used as a positive control.
ELISA: 1:2,000-1:10,000 Dilution.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 486-499 of Human CDC20 (fizzy) (C-terminal) coupled to KLH.
Specificity This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration.
This product reacts with human CDC20 (fizzy).
Cross reactivity may also occur with CDC20 from other sources.
Formulation State: Serum
State: Liquid (sterile filtered) with 0.01% (w/v) Sodium Azide as preservative.
Concentration lot specific
Purification Delipidation and defibrination.
Conjugation Unconjugated
Storage Condition Store vial at -20°C prior to opening.
Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
For extended storage aliquot contents and freeze at -20°C or below.
Avoid cycles of freezing and thawing.
Gene Name cell division cycle 20
Background CDC20, also known as fizzy, Cell division cycle protein 20 homolog, and p55CDC is required for full ubiquitin ligase activity of the anaphase promoting complex/cyclosome (APC/C) and may confer substrate specificity upon the complex. CDC20 appears to act as a regulatory protein interacting with several other proteins at multiple points in the cell cycle. It is required for two microtubule-dependent processes, nuclear movement prior to anaphase and chromosome separation. CDC20 is regulated by MAD2L1. In metaphase the MAD2L1-CDC20-APC/C ternary complex is inactive and in anaphase the CDC20-APC/C binary complex is active in degrading substrates. The phosphorylated form of CDC20 interacts with APC/C. Synthesis is initiated at G1/S, protein level peaks in M phase and protein is abruptly degraded at M/G1 transition.
Synonyms p55CDC
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...