c-Myc (MYC) Rabbit Polyclonal Antibody

CAT#: R1182B

c-Myc (MYC) rabbit polyclonal antibody, Biotin



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CNY 7,181.00


货期*
4周

规格
    • 100 ug

Product images

Specifications

Product Data
Applications ELISA, IHC, WB
Recommend Dilution ELISA: 1/4,000 - 1/20,000.
Western Blot: 1/400 - 1/2,000.
Immunohistochemistry: 1/400 - 1/2,000.
Host Rabbit
Clonality Polyclonal
Immunogen Purified from whole rabbit serum prepared by repeated immunizations with Myc epitope tag peptide E-Q-K-L-I-S-E-E-D-L conjugated to KLH using maleimide. The sequence corresponds to aa 410-419 of human c-Myc.
Specificity This antibody is directed against human c-myc.
Formulation 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 10 mg/ml BSA as stabilizer and 0.01% (w/v) Sodium Azide as perservative
Label: Biotin
State: Lyophilized
Label: Biotinamidocaproate N-Hydroxysuccinimide Ester (BAC)
Molar radio: 10-20 BAC molecules per Rabbit IgG molecule
Reconstitution Method Restore with 0.1 ml of deionized water (or equivalent).
For extended storage mix with glycerol to 50%.
Concentration lot specific
Purification Affinity chromatography
Conjugation Biotin
Storage Condition Prior to reconstitution store at 2-8°C.
Following reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name v-myc avian myelocytomatosis viral oncogene homolog
Background Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.
Synonyms myc tag, myc-tag, c-myc tag
Reference Data
Protein Families Druggable Genome, Embryonic stem cells, Induced pluripotent stem cells, Stem cell - Pluripotency, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - TGFb/BMP signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway, Transcription Factors
Protein Pathways Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Jak-STAT signaling pathway, MAPK signaling pathway, Pathways in cancer, Small cell lung cancer, TGF-beta signaling pathway, Thyroid cancer, Wnt signaling pathway
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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