KTI3 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. ELISA: 1/5,000-1/20,000. This product has been assayed against 1.0 µg of Trypsin inhibitor [soy bean] in a standard ELISA using peroxidase conjugated affinity purified anti-rabbit IgG (goat) and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/3,000 to 1/12,000 of the reconstitution concentration is suggested.
Reactivity	Soy Bean
Host	Rabbit
Clonality	Polyclonal
Immunogen	Trypsin inhibitor from soy bean
Specificity	This antibody detects soy bean Trypsin inhibitor. Cross reactivity against Trypsin inhibitor from other sources may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-rabbit serum as well as purified and partially purified Trypsin inhibitor [soy bean].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium hloride, pH 7.2 State: Ig Fraction State: Lyophilized IgG fraction Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water or equivalent.
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P01070
Background	Soybean trypsin inhibitor inhibits trypsin and to a lesser extent chymotrypsin and plasmin. Soybean trypsin inhibitor will also inhibit other proteases by a mechanism similar to trypsin. It has inhibitory effects towards plasma kallikrein and coagulation Factor Xa. However, soybean trypsin inhibitor will not inhibit metalloproteases, tissue-based kallikrein, acid proteases, or thio proteases. Anti-trypsin inhibitor antibody is ideal for investigators involved in cell signaling, biochemistry and signal transduction research.
Reference Data

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