Luciferase (Photinus pyralis) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation, Immunhistochemistry and most Immunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 µg of Luciferase [Firefly] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Goat IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20000 to 1:100000 of the reconstitution concentration is suggested for this product. For Western Blot, a recommended working dilution is 1:2000 - 1:10000.
Host	Goat
Clonality	Polyclonal
Immunogen	Luciferase [Firefly].
Specificity	Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Luciferase [Firefly]. Cross reactivity against Luciferase from other sources has not been determined
Formulation	PBS, pH 7.2 containing 0.01% sodium azides as a preservative. State: Serum State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore with 2.0 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Prepared from monospecific antiserum by a delipidation and defibrination.
Conjugation	Unconjugated
Storage Condition	Store vial at 2-8°C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20°C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Database Link	UniProt ID P08659
Background	Luciferase from the firefly has become one of the more widely used reporter proteins for the study of gene expression. Luciferase catalyzes a bioluminescent reaction which requires the substrate luciferin as well as Mg2+ and ATP. Mixing these reagents with the cell extract containing luciferase, results in a flash of light that decays rapidly. This light can be detected by a luminometer. The total light emission is proportional to the luciferase activity of the sample.
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.