Folate receptor alpha Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IP, WB
Recommend Dilution	Suitable for immunoblotting (Western or dot blot), ELISA, immunoprecipitation and most immunological methods requiring high titer and specificity. Recommended Dilutions: ELISA: 1/1,000-1/2,500 Western blot: 1/500-1/1,000 Immunoprecipitation: 1/100 This product has been assayed against 1.0 µg of folate binding protein/ Folate receptor alpha [bovine milk] in a standard sandwich ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
Reactivity	Bovine
Host	Goat
Clonality	Polyclonal
Immunogen	Folate binding protein / folate receptor alpha from bovine milk
Specificity	This antibody detects bovine folate receptor alpha. Cross reactivity against FR-alpha from other sources is unknown. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-goat serum as well as purified and partially purified folate binding protein [bovine milk].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: with 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Multi-step process including delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P02702
Background	Folate binding protein, has a high affinity for folic acid and for several reduced folic acid derivatives and mediates delivery of 5 methyltetrahydrofolate to the interior of cells. Membrane bound and soluble forms of a high affinity folate binding protein have been found in kidney, placenta, serum, milk, and in several cell lines. The 2 forms have similar binding characteristics for folates, are immunologically crossreactive, and, based upon limited amino acid sequence data, are nearly identical. There may be a precursor product relationship between the membrane and soluble forms, the membrane form having additional amino acid residues and greater molecular weight. The membrane form has been shown to mediate the transport of folate in cells grown in physiologic concentrations of folate. A function for the soluble form, which is found in serum, milk, and urine, has not been identified.
Synonyms	FR-alpha, Folate receptor 1, MOv18, KB cells FBP, FOLR1, FOLR
Reference Data

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