Alkaline phosphatase / ALPI / IAP Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Western Blot: 1/500-1/2,000. ELISA: 1/4,000-1/20,000. This antibody has been assayed against 1.0 µg of Alkaline phosphatase in a standard capture ELISA using peroxidase conjugated streptavidin and ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000 to 1/20,000 of the reconstitution concentration is suggested.
Reactivity	Bovine
Host	Rabbit
Clonality	Polyclonal
Immunogen	Alkaline phosphatase from calf intestine
Specificity	This product detects bovine Alkaline phosphatase. Cross reactivity against Alkaline phosphatase from other tissues and species may occur but have not been specifically determined. Immunoelectrophoresis give a single precipitin arc against anti-biotin, anti-rabbit serum as well as purified and partially purified Alkaline phosphatase [calf intestine].
Formulation	0.02 M Potassium phosphate, 0.15 M Sodium chloride, pH 7.2 Label: Biotin State: Purified State: Lyophilized purified Ig fraction Stabilizer: 10 mg/ml BSA (immunoglobulin and protease free) Preservative: 0.01% (w/v) Sodium azide
Reconstitution Method	Restore with 0.1 ml of deionized water (or equivalent).
Concentration	lot specific
Purification	Delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Database Link	UniProt ID P19111
Synonyms	Intestinal alkaline phosphatase
Reference Data

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