Apolipoprotein B (APOB) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	Anti-apoLipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and for Western blotting for highly sensitive analysis. Recommended Dilutions: ELISA: 1/2,000-1/10,000. Western blot: 1/200-1/1,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/500.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	apoLipoprotein Type B isolated from Human plasma by density gradient centrifugation followed by HPLC purification.
Specificity	This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other ApoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with Human ApoLipoprotein B and has negligible cross-reactivity with Type A-I, A-II, C-I, C-II, C-III, E and J ApoLipoproteins. Non-specific cross reaction of anti-apoLipoprotein antibodies with other Human serum proteins is negligible.
Formulation	0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0, with 0.01% (w/v) Sodium Azide as preservative. State: Purified State: Liquid (sterile filtered) purified Ig fraction.
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody at 2-8°C prior to opening. Dilute only prior to use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing.
Gene Name	apolipoprotein B
Database Link	Entrez Gene 338 Human UniProt ID P04114
Background	Lipids (triglyceride (TG), cholesterol (Chol), phospholipids (PL), cholesteryl esters (CE)) are insoluble in plasma. These lipids combine with apolipoproteins to form lipoproteins. This process solubilizes lipids in plasma. Lipoproteins provide a transport system for lipids. They also play an important role in lipoprotein receptor recognition and regulation of certain enzymes in lipoprotein metabolism. Apolipoprotein B plays an essential role in lipid transport and metabolism. Apo B may regulate cholesterol synthesis through its interaction with specific cell membrane receptors and by inhibition of HMG Co A reductase. This enzyme has been identified as the rate controlling enzyme in cholesterol biosynthesis. Apo B may be important in the genesis of atherosclerosis. Apo B is the major protein moiety of all lipoproteins other than high density lipoprotein (HDL) in plasma. Increased levels of apo B100 are associated with an increased risk of coronary artery disease. Apo B exists in human plasma as two isoforms: apo B48 and apo B100. Apo B100 is the major physiological ligand for the LDL receptor. It contains 4536 amino acid residues (Chen et al., 1986; Law et al., 1986). Apo B100 is the full length protein. Its gene has been mapped on the short arm of chromosome 2, with an approximate length of 43 kilobases and 29 exons (Ludwig, et al., 1987). The LDL-binding domain of the molecule is proposed to be located between the aa3129-3532 (Knott, et al., 1986). Apo B100 is synthesised in the liver. It is required for the assembly of very low density lipoproteins (VLDL).
Synonyms	ApoB, Apo-B, ApoB100, ApoB-100
Reference Data

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