Apolipoprotein A II (APOA2) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, IP, WB
Recommend Dilution	This Apo AII antibody R1031P has been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for Immunoprecipitation and Western blotting for highly sensitive qualitative analysis. Recommended Dilutions: ELISA: 1/5,000-1/10,000. Immunoprecipitation: 1/100. Western blot: 1/5,000-1/10,000. Immunohistochemistry: 1/50-1/200.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	ApoLipoprotein Type A-II was isolated from Human plasma by Density Gradient Centrifugation followed by HPLC purification.
Specificity	This product has been prepared by Immunoaffinity Chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with ApoLipoprotein A-II and has negligible cross-reactivity with Type A-I, B, C-I, C-II, C-III, E and J apoLipoproteins. Non-specific cross reaction of anti-apoLipoprotein antibodies with other Human serum proteins is negligible.
Formulation	0.125M Sodium Borate, 0.075M Sodium Chloride, 0.005M EDTA, pH 8.0 and 0.01% Sodium Azide as preservative. State: Purified State: Liquid (sterile filtered) purified Ig fraction
Concentration	lot specific
Purification	Immunoaffinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (mix with an equal volume of glycerol and aliquot) at -20°C for longer. Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
Gene Name	apolipoprotein A2
Database Link	Entrez Gene 336 Human UniProt ID P02652
Background	Apolipoprotein A II is the second most abundant protein of the high density lipoprotein particles. The apolipoprotein A II gene consists of 4 exons and 3 introns. The four exons encode the 5' untranslated region, pre peptide, a short N terminal domain and a C terminal domain composed of a variable number of lipid binding amphipathic helices. Familial apolipoprotein A II deficiency may result from a splice junction alteration which blocks splicing of intron 3 from the primary transcript and result in the formation of a non functional mRNA.
Synonyms	Apolipoprotein A-II, Apolipoprotein A2, Apo-AII, ApoA-II, APOA2
Reference Data

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