IKB alpha (NFKBIA) (C-term) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, IHC, WB
Recommend Dilution	Suitable for Immunoblotting (1/2,000-1/10,000), ELISA (1/20,000-1/100,000) and Immunohistochemistry (1/1,000-1/5,000). Recommended Dilutions: This product was assayed by immunoblot and found to be reactive against IκBa showing a 36 kDa band. Perform all incubations except color development using TBS supplemented with 0.1% Tween-20 at room temperature. Block the membrane with 5% dry milk for 2 h. Add a 1:1,000 dilution of the primary antibody to the membrane and incubated for 2 h. Perform washes with buffer 4 times for 5' each. Incubate the immunoblot with Peroxidase conjugated Affinity Purified Goat anti-Rabbit IgG [H&L] at 1/2,000 for 1 h. Wash with TBS only preceded color development.
Reactivity	Human, Mouse, Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	IkBa peptide corresponding to a region near the C-terminus of the Human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity	This antibody may react non-specifically with other proteins. This antibody is suitable for the detection by immunoblot of Human, Mouse and Rat IκB-alpha. Control Peptide (R1013CP) will compete only with the specific reaction of antiserum with IκB-alpha.
Formulation	0.02M Potassium Phosphate, 0.15M Sodium Chloride, pH 7.2 containing 0.09% (w/v) Sodium Azide as preservative. State: Serum State: Liquid (sterile filtered) Ig fraction.
Concentration	lot specific
Purification	Prepared from monospecific antiserum by Delipidation and Defibrination.
Conjugation	Unconjugated
Storage Condition	Store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Dilute only prior to immediate use. Avoid repeated freezing and thawing.
Gene Name	NFKB inhibitor alpha
Database Link	Entrez Gene 4792 Human UniProt ID P25963
Background	Three major forms of IKB like molecules have been identified and each is characterised by multiple copies of ankyrin repeats. IKB alpha and IKB beta appear to be the major regulatory forms of IKB in most cells. These proteins interact with p65 or cRel containing forms of NFkB and block nuclear import by masking the nuclear localisation sequences of NFkB. The activation of NFkB involves the inducible phosphorylation and subsequent degradation of IKB. Immunoblotting easily detects the hyperphosphorylated forms of IKB alpha, but not phosphorylated IKB beta. Interestingly, IKB alpha and IKB beta mediate different NFkB responses. IkB alpha appears to control more transient activation of NFkB in response to an inducer, while IKB beta controls a persistent response. Bcl3 interacts with p50 and p52 containing forms of NFkB, but rather than being an inhibitor it appears to function to stimulate transcription. The degradation of IKB is confirmed by immunoblotting.
Synonyms	I-kappa-B-alpha, MAD3, NFKBI, I kappa B-alpha, IkappaBalpha, IkB-alpha
Reference Data

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