beta Defensin 3 (DEFB103A) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human BD-3 by Direct ELISA (using 100µl/well) a concentration of ~1.0 µg/ml of this antibody is required. This Biotinylated antibody allows the detection of at least 0.2-0,4 mg/well of recombinant Humand BD-3. Sandwich ELISA: To detect Human BD-3 by Sandwich ELISA (using 100µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. This Biotinylated antibody, in conjugation with Purified Anti-Human BD-3 antibody (Cat.-No PP1229P) as a Capture antibody, allows the detection of at least 0.2-0.4 mg/well of recombinant Humand BD-3. Western blot: To detect Human BD-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human BD-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	E.coli derived recombinant Human Beta Defensin-3 (Cat.-No PA184).
Specificity	This antibody reacts with Human BD3 (Defensin beta 3). Other species not tested.
Formulation	BPS, pH 7.2 without preservatives Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	defensin beta 103A
Database Link	Entrez Gene 414325 Human UniProt ID P81534
Background	Beta Defensin 3 exhibits antimicrobial activity against Gram-positive bacteria S.aureus and S.pyogenes, Gram-negative bacteria P.aeruginosa and E.coli and the yeast C.albicans. It kills multiresistant S.aureus and vancomycin-resistant E.faecium, but it appears to have no significant hemolytic activity. Beta Defensin 3 is highly expressed in skin and tonsils, and to a lesser extent in trachea, uterus, kidney, thymus, adenoid, pharynx and tongue. Low expression is observed in salivary gland, bone marrow, colon, stomach, polyp and larynx, with no expression in small intestine. Expression is induced by infection of bacteria and by interferon gamma. Beta Defensin 3 belongs to the beta-defensin family.
Synonyms	DEFB103A, DEFB103B, BD3, DEFB3, HBP3, HBD3, DEFB103, Beta-defensin 103, Defensin-like protein, Defensin beta 103
Note	Molecular weight: ~150 kDa
Reference Data

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