TL1A (TNFSF15) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect hTL-1A by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at ~1.0 µg/ml. This biotinylated polyclonal antibody allows the detection of at least 0.2–0.4 ng/well of recombinant hTL-1A. Sandwich ELISA: To detect hTL-1A by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25 – 1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with purified Anti-Human TL-1A (Cat.-No PP1228P) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of recombinant hTL-1A. Western blot: To detect hTL-1A by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTL-1A is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant hTL-1A (human TL-1A).
Specificity	Recognizes Human TL-1A. Other species not tested.
Formulation	PBS, pH 7.2 with containig no preservative or stabilizer. Label: Biotin State: Lyophilized (Sterile filtered) purified Ig fraction
Reconstitution Method	Centrifuge vial prior to opening. Restore with sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month  or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor superfamily member 15
Database Link	Entrez Gene 9966 Human UniProt ID O95150
Synonyms	TL1, TL1A, VEGI
Reference Data

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