Il22 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA Indirect: To detect mIL-22 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant mIL-22. Sandwich: To detect mIL-22 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine IL-22 (PP1226B1 or PP1226B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mIL-22. Western blot To detect mIL-22 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mIL-22 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant mIL-22 (murine Interleukin-22).
Specificity	This antibody recognizes murine IL-22.
Formulation	PBS, pH 7.4 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of > 0.2 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	The lyophilized antibody is stable at -20°C for one year from despatch. The reconstituted antibody can be stored for two weeks at 2-8°C. Frozen aliquots are stable for six months when stored at -20°C. Avoid repeated freezing and thawing.
Gene Name	interleukin 22
Database Link	Entrez Gene 50929 Mouse UniProt ID Q9JJY9
Background	IL22 is a cytokine structurally related to IL10 and is upregulated in activated T cells. IL22 signals through a receptor complex that includes CRF2-4 and a new member of the class II cytokine receptor family (also known as the interferon receptor family), IL22R. CRF2-4 has previously been demonstrated to be a functional component of the IL-10 signaling complex. IL22 is able to suppress IL4 production; this may have therapeutic potential, particularly in the treatment of asthma.
Synonyms	IL-22, ILTIF, Cytokine Zcyto18
Note	Centrifuge vial prior to opening!
Reference Data

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