TNFRSF1B Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect hsTNF Receptor II by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. This Biotin conjugated antibody allows the detection of at least 0.2-0.4 ng/well of recombinant hsTNF Receptor II. Sandwich ELISA: To detect hsTNF-receptor II by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This Biotin conjugated antibody, in conjunction with Affinity purified Anti-Human sTNF-receptor II (Cat.-No PP1222P) as a captor antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hsTNF-receptor II. Western blot: To detect hsTNF Receptor II by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hsTNF Receptor II is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly purified (>98%) E. coli derived recombinant hsTNF Receptor II (Human soluble Tumor Necrosis Factor Receptor II).
Specificity	This antibody recognizes Human soluble TNF Receptor II. Other species not tested.
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized Sterile filtered purified Ig fraction Label: conjugated
Reconstitution Method	Restore to a concentration of 0.1-1 mg/ml with sterile PBS solution containing 0.1% BSA.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor receptor superfamily member 1B
Database Link	Entrez Gene 7133 Human UniProt ID P20333
Background	Tumor Necrosis Factor (TNF) is a cytokine whose function is mediated through two distinct cell surface receptors (TNF Receptor I and TNF Receptor II) that are included in the TNF receptor superfamily along with FAS antigen and CD40. TNF receptors I and II are membrane glycoproteins and they are from the family of cell surface molecules including nerve growth factor receptor, Fas/Apo1, CD30, OX40, and 4-1BB, which are characterized by cysteine rich motifs in the extracellular domain. TNF Receptor II (p75, CD120b) is present on most cell types (including monocytes, endothelial cells, Langerhans cells, and macrophages) and is considered to play a role in cell stimulation by TNF alpha. TNF Receptor II molecule is shown to be responsible for stimulation of activated T lymphocytes by TNF alpha.
Synonyms	Tumor necrosis factor receptor 2, p80 TNF-alpha receptor, TNFRSF1B, TNFBR, TNF-R2
Reference Data

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