Kitlg Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, Neutralize, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat SCF (50.0 ng/ml), a concentration of 1.2-1.8 μg/ml of this antibody is required. Indirect ELISA: To detect Rat SCF by indirect ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat SCF. Sandwich ELISA: To detect Rat SCF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotin conjugated Anti-Rat SCF (PP1216B1 or PP1216B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat SCF. Western Blot: To detect Rat SCF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat SCF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Rat
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant Rat SCF.
Specificity	Recognizes Rat SCF
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method	Centrifuge vial prior to opening. Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing an immobilized Rat SCF matrix.
Conjugation	Unconjugated
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	KIT ligand
Database Link	Entrez Gene 60427 Rat UniProt ID P21581
Synonyms	KITL, Kit ligand, c-Kit ligand, Stem cell factor, Mast cell growth factor, MGF
Reference Data

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