Il3 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Rat IL-3beta (100 ng/ml), a concentration of 2.7-4.0 µg/ml of this antibody is required. ELISA: To detect rat IL-3 beta by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.15-0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant rat IL-3 beta. Western Blot: To detect rat IL-3 beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant rat IL-3 beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Rat
Host	Goat
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant rIL-3 beta.
Specificity	Rat Interleukin-3 beta
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml
Purification	Affinity chromatography
Conjugation	Unconjugated
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	interleukin 3
Database Link	Entrez Gene 24495 Rat UniProt ID P97688
Background	IL3 acts in hematopoiesis by controlling the production, differentiation, and function of two related white cell populations of the blood, the granulocytes and the monocytes/macrophages. It induces granulocytes, macrophages, mast cells, stem cells, erythroid cells, eosinophils and megakaryocytes and is expressed by activated T cells, mast cells, and natural killer cells.
Synonyms	IL-3 beta, IL3B
Note	Centrifuge vial prior to opening!
Reference Data

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