TNFSF12-TNFSF13 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: (Direct): To detect hTWEAK by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hTWEAK. (Sandwich): To detect hTWEAK by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Human TWEAK PP1124P as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant hTWEAK. Western Blot: To detect hTWEAK by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTWEAK is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant hTWEAK.
Specificity	Human TWEAK
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized purified Ig fraction Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	TNFSF12-TNFSF13 readthrough
Database Link	Entrez Gene 407977 Human UniProt ID O43508
Synonyms	APO3 ligand, APO3L, DR3LG
Note	Centrifuge vial prior to opening!
Reference Data

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