FGF10 Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA To detect Human FGF-10 (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. In conjunction with compatible secondary reagents, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-10. Sandwich ELISA: To detect Human FGF-10 (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. In conjunction with Polyclonal Anti-Human FGF-10 (PP1119P) as a capture antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant hFGF-10. Western blot: To detect Human FGF-10 this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-10 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	E. Coli derived recombinant Human FGF-10
Specificity	This antibody detects FGF-10.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (Sterile filtered) Ig fraction.
Reconstitution Method	Restore in sterile PBS containing 0.1 % BSA to a concentration of 0.1 - 1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store the lyophilized antibody at -20°C. Following reconstitution it is stable for two weeks at 2-8°C. Frozen aliquots are stable for 6 months when stored at -20°C. Avoid repeated freezing and thawing.
Gene Name	fibroblast growth factor 10
Database Link	Entrez Gene 2255 Human UniProt ID O15520
Background	FGF10 is a heparin binding growth factor that stimulates the proliferation and activation of cells that express FGF receptors. FGF10 is mostly related to FGF-7/KGF and is expressed during development and preferentially in adult lungs.
Synonyms	Fibroblast growth factor 10, FGF-10, Keratinocyte growth factor 2, KGF2
Note	Centrifuge vial prior to opening.
Reference Data

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