Vegfa Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Murine VEGF (50.0 ng/ml), a concentration of 1.0-2.0 µg/ml of this antibody is required. Sandwich ELISA: To detect Murine VEGF by Sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antibody, in conjunction with biotinylated Anti-Murine VEGF (PP1113B) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine VEGF. Western Blot: To detect Murine VEGF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mVEGF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry on Paraffin Sections: 2.5 µg/ml for 1h at RT (Previous Lots). Staining of Formalin-Fixed, Paraffin-Embedded Sections of normal Murine Kidney using a secondary Fluorophore conjugated antibody for 30 min at RT. High pH heat induced antigen retrieval is recommended.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	E.coli derived highly pure (> 98%) recombinant Murine VEGF
Specificity	Reacts with Vascular Endothelial Growth Factor (VEGF).
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	vascular endothelial growth factor A
Database Link	Entrez Gene 22339 Mouse UniProt ID Q00731
Background	VEGF (vascular endothelial growth factor) is a homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. It exhibits potent mitogenic and permeability inducing properties specific for the vascular endothelium. Of the four isoforms of VEGF, the smaller two, VEGF165 and VEGF121, are secreted proteins and act as diffusible agents, whereas the larger two (VEGF189 and VEGF206) remain cell associated.
Synonyms	VEGFA, VEGF, VPF, Vascular endothelial growth factor A, Vascular permeability factor
Note	Centrifuge vial prior to opening!
Reference Data

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