alpha Defensin 1 (DEFA1) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA
Recommend Dilution	Direct ELISA: To detect Human NP-1 by Direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. This Biotin conjugated antibody allows the detection of at least 0.2 ng/well of recombinant Human NP-1. Sandwich ELISA: To detect Human NP-1 by Sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. This Biotin conjugated antibody in conjunction withPurified antibody PP1112P, allows the detection of at least 0.2 ng/well of recombinant Human NP-1. Western Blot: To detect Human NP-1 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hNP-1 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived Human recombinant NP-1.
Specificity	Defensin alpha 1-3 (NP-1)
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	defensin alpha 1
Database Link	Entrez Gene 728358 HumanEntrez Gene 1667 Human UniProt ID P59665
Background	Defensins are a group of cyclic peptides containing 29-35 amino acids, which comprise approximately 30% of the total protein content of neutrophil azurophilic granules. Various functions have been reported for the defensins, including antibacterial and antifungal activity and chemotactic activity for monocytes.
Synonyms	DEFA1, DEF-A1, DEFA-1, DEF1, DEFA2, MRS, DEF-1, DEFA-2, DEF-A2, HNP-1, HP-1, HP1, HNP1, Neutrophil defensin 1, DEFA3, DEFA-3, DEF-A3, DEF3, DEF-3, HNP-3, HP-3, HP3, HNP3, Neutrophil defensin 3, Defensin alpha 1, Defensin alpha 3
Note	Centrifuge vial prior to opening!
Reference Data
Protein Families	Druggable Genome

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