SCGF (CLEC11A) (beta) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human SCGF-Beta by Direct ELISA (using 100 µl/well) a concentration of ~1.0 µg/ml of this antibody is required. This Biotinylated antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human SCGF-Beta. Sandwich ELISA: To detect Human SCGF-Beta by Sandwich ELISA (using 100 µl/well) this antibody can be used at a concentration of 0.25-1.0 µg/ml. Used in conjunction with purified anti-Human SCGF beta antibody (PP1106P1 or PP1106P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human SCGF-Beta. Western Blot: To detect Human SCGF-Beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCGF-Beta is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant human SCGF-beta.
Specificity	Stem Cell Growth Factor beta (SCGF-beta)
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-type lectin domain family 11 member A
Database Link	Entrez Gene 6320 Human UniProt ID Q9Y240
Background	This cytokine stimulates the proliferation and differentiation of hematopoietic precursor cells from various lineages, including erythrocytes, lymphocytes, granulocytes and macrophages. It acts synergistically with other cytokines, including IL3, GCSF, GMCSF and FLT3 ligand and suppresses SCF-stimulated erythrocyte proliferation.
Synonyms	Stem cell growth factor, CLECSF3, LSLCL
Note	Centrifuge vial prior to opening!
Reference Data
Protein Families	Druggable Genome, Secreted Protein

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