IL12A (alpha + beta) Goat Polyclonal Antibody
CAT#: PP1102P1
IL12A (alpha + beta) goat polyclonal antibody, Aff - Purified
Conjugation: Biotin
Need it in bulk or conjugated?
Get a free quote
CNY 3,910.00
货期*
5周
规格
Product images
![](https://cdn.origene.com/img/defaults-img.jpg)
Specifications
Product Data | |
Applications | ELISA, FN, WB |
Recommend Dilution | Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-12 (0.3 ng/ml), a concentration of 0.012 - 0.02 µg/ml of this antibody is required. ELISA: To detect hIL-12 by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hIL-12. Western Blot: To detect hIL-12 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-12 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Human |
Host | Goat |
Clonality | Polyclonal |
Immunogen | Highly pure (>98%) recombinant hIL-12 (human Interleukin-12) |
Specificity | Interleukin-12 (IL-12) |
Formulation | PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction. |
Reconstitution Method | Restore in sterile water to a concentration of 0.1-1.0 mg/ml. |
Concentration | N/A |
Purification | Affinity chromatography. |
Conjugation | Unconjugated |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Gene Name | interleukin 12A |
Database Link | |
Background | Interleukin 12 (IL12) can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine activated killer cells, and stimulate the production of IFN gamma by resting PBMC. IL12 induces IFN gamma, TNF alpha production in T and NK cells; costimulation of PBL proliferation; proliferation/differentiation of TH1 T lymphocytes. It is downregulated by IL10; homodimeric p40 antagonistic to functional p70 heterodimer. IL12R beta 1 binds p40 but when dimeric with IL12R beta 2 binds p35. |
Synonyms | IL-12A, IL-12 subunit p35, NKSF1, CLMF p35 |
Note | Centrifuge vial prior to opening1 |
Reference Data | |
Protein Families | Druggable Genome, Secreted Protein |
Protein Pathways | Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus |
Documents
Product Manuals |
FAQs |
SDS |
Resources
抗体相关资料 |
Customer
Reviews
Loading...