LEC (CCL16) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: To detect hLEC by direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hLEC. Western Blot: To detect hLEC by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hLEC is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant hLEC.
Specificity	Human LEC
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 16
Database Link	Entrez Gene 6360 Human UniProt ID O15467
Background	LEC (liver expressed cytokine) displays chemotactic activity for lymphocytes and monocytes but not for neutrophils. It also shows a potent myelosuppressive activity and suppresses proliferation of myeloid progenitor cells. Its expression is upregulated by IL10.
Synonyms	C-C motif chemokine 16, Chemokine LEC, Liver-expressed chemokine, Monotactin-1, Chemokine CC-4, LCC1, LCC-1, NCC-4, ILINCK, SCYA16
Note	Centrifuge vial prior to opening!
Reference Data

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