Macrophage Inflammatory Protein 3 (CCL23) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human MIP-3 by Direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1.0 µg/ml. It allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-3. Sandwich ELISA: To detect Human MIP-3 by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjuction with anti-Human MIP-3 (Cat.-No PP1089P1/P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIP-3. Western Blot: To detect Human MIP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIP-3 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human MIP-3.
Specificity	The antibody reacts with Macrophage Inflammatory Protein-3.
Formulation	PBS, pH 7.2 Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method	Centrifuge the vial prior to opening. Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	The lyophilized antibody is stable at -20°C for one year from date of despatch. Reconstituted antibody is stable for 2 weeks at 2-8°C and for six months when stored in working aliquots at -20°C. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 23
Database Link	Entrez Gene 6368 Human UniProt ID P55773
Background	Macrophage Inflammatory Protein 3 (MIP3) is chemotactic for monocytes, resting T-lymphocytes, and neutrophils. It inhibits proliferation of myeloid progenitor cells and is highly expressed in adult lung, liver, skeletal muscle and pancreas. There are moderate levels of MIP3 in fetal liver, adult bone marrow and placenta. MIP3 belongs to the intercrine beta family (small cytokine c-c) (chemokine c-c).
Synonyms	C-C motif chemokine 23, CCL-23, MIP-3, MPIF1, CK-beta-8, CKB-8, Small-inducible cytokine A23, SCYA23
Reference Data

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