SDF1 (CXCL12) (beta) Goat Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Sandwich ELISA: To detect Human SDF-1 beta by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. PP1068P antibody, in conjunction with Biotinylated Anti-Human SDF-1 beta (PP1068B1 or PP1068B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human SDF-1 beta. Western Blot: To detect Human SDF-1 beta by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1 bet is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Goat
Clonality	Polyclonal
Immunogen	E. Coli derived Recombinant recombinant Human SDF-1 beta
Specificity	Reacts with Stromal Cell Derived Factor-1 beta (SDF-1 beta).
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Centrifuge vial prior to opening! Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody prior to reconstitution at -20°C. ollowing reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-X-C motif chemokine ligand 12
Database Link	Entrez Gene 6387 Human UniProt ID P48061
Background	SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.
Synonyms	Stromal cell-derived factor 1, SDF1A, SDF1B, IRH, hIRH, PBSF
Note	Molecular Weight: ~150 kDa
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Axon guidance, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Leukocyte transendothelial migration

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