SDF1 (CXCL12) (alpha) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of Human SDF1-alpha (100 ng/ml), a concentration of 2-4 µg/ml of this antibody is required. Sandwich ELISA: Using 100 μl/well antibody solution, a concentration of 0.5-2.0 μg/ml is required. In conjunction with Biotinylated Anti-Human SDF1-alpha (Cat.-No PP1067B) as a detection antibody, it allows the detection of at least 0.2-0.4 ng/well of recombinant Human SDF1-alpha. Western Blot: 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSDF-1α is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: 0.5 µg/ml with an overnight incubation at 4°C. Staining of Formalin-Fixed, Paraffin-Embedded sections of Human colorectal adenocarcinoma using a HRP-labeled polymer detection sytem with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 Sodium citrate buffer is recommended.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human SDF1-alpha (Cat.-No PA126)
Specificity	This antibody detects Human Stromal Cell Derived Factor-1 alpha. Other species not tested.
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing immobilized Human SDF1a matrix.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	C-X-C motif chemokine ligand 12
Database Link	Entrez Gene 6387 Human UniProt ID P48061
Background	SDF1 alpha and 1 beta are small cytokines that belong to the intercrine family, members of which activate leukocytes and are often induced by proinflammatory stimuli such as lipopolysaccharide, TNF or IL1. SDF1 alpha has been shown to be chemotactic for lymphocytes and is recently reported to be a ligand for CXCR4 (LESTR/fusin), a co-receptor for HIV1 entry into T cells. SDF-1 binding to CXCR4 inhibits HIV1 entry.
Synonyms	Stromal cell-derived factor 1, SDF1A, SDF1B, IRH, hIRH, PBSF
Note	Centrifuge vial prior to opening!
Reference Data
Protein Families	Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways	Axon guidance, Chemokine signaling pathway, Cytokine-cytokine receptor interaction, Leukocyte transendothelial migration

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