SCF (KITLG) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hSCF (10 ng/ml), a concentration of 8 - 12 ng/ml of this antibody is required. ELISA: To detect hSCF by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hSCF. Western Blot: To detect hSCF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hSCF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Immunohistochemistry: 1 µg/ml is the recommended starting dilution for frozen and paraffin sections. For paraffin sections antigen retrieval techniques may be necesserary to improve staining.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant hSCF (anti-hSCF).
Specificity	Human Stem Cell Factor (SCF).
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	KIT ligand
Database Link	Entrez Gene 4254 Human UniProt ID P21583
Synonyms	KITL, Kit ligand, c-Kit ligand, Stem cell factor, Mast cell growth factor, MGF
Note	Centrifuge vial prior to opening!
Reference Data
Protein Families	Druggable Genome, Transmembrane
Protein Pathways	Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Melanogenesis, Pathways in cancer

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