PF4 Rabbit Polyclonal Antibody

CAT#: PP1063P2

PF4 rabbit polyclonal antibody, Aff - Purified

Conjugation: Unconjugated Biotin



Need it in bulk or conjugated?
Get a free quote

CNY 4,790.00


货期*
5周

规格
    • 100 ug

Product images

Specifications

Product Data
Applications ELISA, FN, IHC, WB
Recommend Dilution Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hPF-4 (50 ng/ml), a concentration of 1.75-3.0 µg/ml of this antibody is required.
Direct ELISA: To detect hPF-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant hPF-4. 
Sandwich ELISA:
To detect hPF-4 by Sandwich ELISA (using 100µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human PF-4 (Cat.-No PP1063B) as a Detection antibody, allows the detection of 0.2-0.4 ng/well of recombinant hPF-4. 
Western Blot: To detect hPF-4 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hPF-4 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. 
Immunohistochemistry: The recommended concentration is 1.0-2.0 µg/ml with an overnight incubation at 4°C. An alkaline phosphatase-labeled polymer detection system was used with a red chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) E.coli derived recombinant human Platelet Factor-4 (hPF-4)
Specificity This antibody reacts with Human Platelet Factor 4 (PF-4).
Formulation PBS, pH 7.2 without preservatives
State: Aff - Purified
State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method
Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography employing immobilized Human PF-4 matrix
Conjugation Unconjugated
Storage Condition

Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month 
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.

Gene Name platelet factor 4
Synonyms PF-4, Iroplact, Oncostatin-A, CXCL4, SCYB4
Note Centrifuge vial prior to opening.
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...