PDGF AA (PDGFA) (PDGF-AA) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human PDGF-AA by Direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~ 1.0 µg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant Human PDGF-AA. Sandwich ELISA: To detect Human PDGF-AA by Sandwich ELISA (using 100 μl/well antibody solution), a concentration of 0.25-1.0 μg/ml of this antibody is required, in conjunction with a purified anti-Human PDGF-AA (Cat.-No PP1061P) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human PDGF-AA. Western Blot: To detect Human PDGF-AA by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. When Used in conjunction with compatible secondary reagents the detection limit for recombinant Human PDGF-AA is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human PDGF-AA (Cat.-No PA118)
Specificity	Human Platelet Derived Growth Factor AA (hPDGF-AA).
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	platelet derived growth factor subunit A
Database Link	Entrez Gene 5154 Human UniProt ID P04085
Background	PDGFs are disulfide-linked dimers consisting of two 12.0-13.5 kDa polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs; PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFRalpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.
Synonyms	Platelet-derived growth factor subunit A, PDGF alpha, PDGF A, PDGF-A, Platelet-derived growth factor alpha, PDGF subunit A, Platelet-derived growth factor A chain, PDGF1, PDGF-1
Note	Centrifuge vial prior to opening!
Reference Data
Protein Families	Druggable Genome
Protein Pathways	Cytokine-cytokine receptor interaction, Focal adhesion, Gap junction, Glioma, MAPK signaling pathway, Melanoma, Pathways in cancer, Prostate cancer, Regulation of actin cytoskeleton

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