Oncostatin M (OSM) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human Oncostatin M (10 ng/ml), a concentration of 0.1-0.175 µg/ml of this antibody is required. ELISA: To detect human Oncostatin M by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant human Oncostatin M. Sandwich ELISA: To detect human Oncostatin M by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotin conjugated anti-Human Oncostatin M (cat.-No PP1060B) as a detection antibody, allows the detection of 0.2-0.4 ng/well of recombinant human Oncostatin M. Western Blot:  Use a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human Oncostatin M is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.  Immunohistochemistry: This antibody stained formalin-fixed paraffin-embedded sections of human breast malignant ductal adenocarcinoma. The recommended concentration is 0.5 μg/ml with an overnight incubation at 4°C. An HRP-labeled polymer detecttion system was used wit DAB chromogen and a proteinase K antigen retrieval.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Human Oncostatin M.
Specificity	Human Oncostatin M.
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography employing immobilized Human Oncostatin M matrix
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Predicted Protein Size	~150 KDa
Gene Name	oncostatin M
Database Link	Entrez Gene 5008 Human UniProt ID P13725
Synonyms	OSM
Note	Centrifuge vial prior to opening!
Reference Data

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