MIG (CXCL9) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human MIG by Direct ELISA (using 100 µl/well antibody solution), this antibody can be used at a concentration of ~1.0 µg/ml. This Biotin conjugated antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIG.  Sanwich ELISA: To detect Human MIG by Sandwich ELISA (using 100 µl/well antibody solution), this antibody can be used at a concentration of 0.25-1.0 µg/ml. This Biotin conjugated antibody in conjunction with Purified anti-Human MIG Antibody (Cat.-No PP1055P) as a Capture Antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human MIG. Western Blot: To detect Human MIG by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human MIG is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived Human recombinant MIG
Specificity	Detects Human Monokine induced by interferon-gamma (MIG).
Formulation	PBS, pH 7.2 without preservatives Label: Biotin State: Lyophilized purified (sterile filtered) Ig fraction Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography
Conjugation	Biotin
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-X-C motif chemokine ligand 9
Database Link	Entrez Gene 4283 Human UniProt ID Q07325
Background	MIG (monokine induced by interferon-gamma), a member of the alpha-chemokine family (CXC) of cytokines, is produced by stimulated monocytes, macrophages and endothelial cells. It signals through the CXCR3 receptor. MIG selectively chemoattracts Th1 lymphocytes, and also exerts other activities including inhibition of tumor growth, angiogenesis, and inhibition of colony formation of hematopoietic progenitors. Human MIG is active on murine cells.
Synonyms	C-X-C motif chemokine 9, CXCL9, CMK, MIG, SCYB9
Note	Centrifuge vial prior to opening!
Reference Data

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