MCP2 (CCL8) Rabbit Polyclonal Antibody

CAT#: PP1045P2

MCP2 (CCL8) rabbit polyclonal antibody, Aff - Purified

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 100 ug

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Specifications

Product Data
Applications ELISA, FN, WB
Recommend Dilution Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hMCP-2 (100.00 ng/ml), a concentration of 12-16 µg/ml of this antibody is required.
ELISA: To detect hMCP-2 by Indirect ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5-0.2 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2-0.4 ng/well of recombinant hMCP-2.
Sandwich: To detect hMCP-2 by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.5-2.0 μg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human MCP-2 (PP1045B1/B2) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hMCP-2.
Western Blot: To detect hMCP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) recombinant hMCP-2.
Specificity Human Macrophage Chemotactic Protein-2 (MCP-2).
Formulation PBS, pH 7.2 without preservatives.
State: Aff - Purified
State: Lyophilized purified Ig fraction.
Reconstitution Method Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification Affinity chromatography.
Conjugation Unconjugated
Storage Condition Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name C-C motif chemokine ligand 8
Background MCP 2 (Monocyte Chemoattractant Protein 2) is a chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils. MCP 2 is important to inflammatory host responses, and is found in the highest concentration in the small intestine and peripheral blood cells.
This protein is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. By recruiting leukocytes to sites of inflammation this cytokine may contribute to tumor-associated leukocyte infiltration and to the antiviral state against HIV infection.
Synonyms MCP-2, CCL-8, C-C motif chemokine 8, Small-inducible cytokine A8, SCYA10, SCYA8, HC14, MCP-2
Note Centrifuge vial prior to opening!
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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