MCP1 (CCL2) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: To detect hMCP-1(MCAF) by direct ELISA (using 100 ml/well antibody solution) this antibody can be used at a concentration of 0.15 - 0.30 g/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2 ng/well of recombinant hMCP-1(MCAF). Western Blot: To detect hMCP-1(MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 mg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hMCP-1(MCAF) is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant hMCP-1/MCAF (human Macrophage/Monocyte chemotactic protein-1).
Specificity	Human Macrophage Chemotactic Protein-1 (MCP-1) (MCAF)
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 2
Database Link	Entrez Gene 6347 Human UniProt ID P13500
Background	This chemotactic factor attracts monocytes and basophils but not neutrophils or eosinophils and augments monocyte anti-tumor activity. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis or atherosclerosis. It may be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis.
Synonyms	C-C motif chemokine 2, SCYA2, MCAF, Small-inducible cytokine A2, MCP-1, HC11, HC-11
Note	Centrifuge vial prior to opening!
Reference Data

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