IGF1 Rabbit Polyclonal Antibody
CAT#: PP1020B1
IGF1 rabbit polyclonal antibody, Biotin
Conjugation: Unconjugated
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CNY 3,910.00
货期*
5周
规格
Specifications
Product Data | |
Applications | ELISA, WB |
Recommend Dilution | Direct ELISA: To detect Human IGF-I by direct ELISA (using 100 µl/well antibody solution) a concentration of ~1.0 µg/ml of this antibody is required. This Biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human IGF-I. Sandwich ELISA: To detect Human IGF-I by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. Used in conjunction with Polyclonal anti-Human IGF-I (Cat.-No PP1020P1 or PP1020P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IGF-I. Western blot: To detect Human IGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Hunman IGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. |
Reactivity | Human |
Host | Rabbit |
Clonality | Polyclonal |
Immunogen | E.coli derived recombinant Human IGF-I (Cat.-No PA071) |
Specificity | This antibody reacts with Human Insulin-like Growth Factor 1. Other species not tested. |
Formulation | PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction. Label: conjugated |
Reconstitution Method | Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
Purification | Affinity Chromatography. |
Conjugation | Biotin |
Storage Condition | Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing. |
Gene Name | insulin like growth factor 1 |
Database Link | |
Background | Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth. |
Synonyms | IGF-I, Somatomedin-C, Mechano growth factor, MGF, IBP1 |
Note | Centrifuge vial prior to opening! |
Reference Data | |
Protein Families | Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein |
Protein Pathways | Dilated cardiomyopathy, Focal adhesion, Glioma, Hypertrophic cardiomyopathy (HCM), Long-term depression, Melanoma, mTOR signaling pathway, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer |
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