IGF1 Rabbit Polyclonal Antibody

CAT#: PP1020B1

IGF1 rabbit polyclonal antibody, Biotin

Conjugation: Unconjugated Biotin



Need it in bulk or conjugated?
Get a free quote

CNY 3,910.00


货期*
5周

规格
    • 25 ug

Product images

Specifications

Product Data
Applications ELISA, WB
Recommend Dilution Direct ELISA: To detect Human IGF-I by direct ELISA (using 100 µl/well antibody solution) a concentration of ~1.0 µg/ml of this antibody is required. This Biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human IGF-I.
Sandwich ELISA: To detect Human IGF-I by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.25-1.0 µg/ml of this antibody is required. Used in conjunction with Polyclonal anti-Human IGF-I (Cat.-No PP1020P1 or PP1020P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human IGF-I.
Western blot: To detect Human IGF-I by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Hunman IGF-I is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Human
Host Rabbit
Clonality Polyclonal
Immunogen E.coli derived recombinant Human IGF-I (Cat.-No PA071)
Specificity This antibody reacts with Human Insulin-like Growth Factor 1.
Other species not tested.
Formulation PBS, pH 7.2 without preservatives.
Label: Biotin
State: Lyophilized (sterile filtered) purified Ig fraction.
Label: conjugated
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification Affinity Chromatography.
Conjugation Biotin
Storage Condition Store lyophilized at 2-8°C for 6 months or at -20°C long term.
After reconstitution store the antibody undiluted at 2-8°C for one month
or (in aliquots) at -20°C long term.
Avoid repeated freezing and thawing.
Gene Name insulin like growth factor 1
Background Insulin is a pancreatic hormone that regulates glucose uptake and the synthesis of protein and fat. The insulin like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth promoting activity. IGF1 (Insulin Like Growth Factor I) is a polypeptide growth factor, which stimulates the proliferation of a wide range of cell types including muscle, bone, and cartilage tissue. IGF1 functions as an autocrine regulator of growth.
Synonyms IGF-I, Somatomedin-C, Mechano growth factor, MGF, IBP1
Note Centrifuge vial prior to opening!
Reference Data
Protein Families Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein
Protein Pathways Dilated cardiomyopathy, Focal adhesion, Glioma, Hypertrophic cardiomyopathy (HCM), Long-term depression, Melanoma, mTOR signaling pathway, Oocyte meiosis, p53 signaling pathway, Pathways in cancer, Progesterone-mediated oocyte maturation, Prostate cancer
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
Customer Reviews 
Loading...