CX3CL1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Neutralization. Sandwich ELISA: To detect Human Fractalkine by Sandwich ELISA (using 100 µl/well antibody solution) a concentration of 0.5-2.0 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human Fractalkine (Cat.-No PP1009B1 or PP1009B2) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human Fractalkine. Western Blot: To detect Human Fractalkine by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFractalkine is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.  Immunohistochemistry on Paraffin Sections: 0.25 µg/ml with an overnight incubation at 4°C. This antibody stained formalin-fixed, paraffin-embedded sections of human pancreas malignant ductal adenocarcinoma. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended. Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (> 98%) E.coli derived recombinant Human Fractalkine (Cat.-No PA057).
Specificity	This antibody is specific to Human Fractalkine.
Formulation	PBS, pH 7.2 without preservatives State: Aff - Purified State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography using an immobilized Human Fractalkine matrix
Conjugation	Unconjugated
Storage Condition	Prior to reconstitution store at 2-8°C. Following reconstitution store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-X3-C motif chemokine ligand 1
Database Link	Entrez Gene 6376 Human UniProt ID P78423
Background	CX3CL1 is a member of the delta chemokine subfamily that contains a unique CX3C cysteine motif near the N-terminal. Unlike other known chemokines, it is a type 1 membrane protein containing a chemokine domain tethered on a long mucin-like stalk. CX3CL1, a leukocyte chemoattractant, is expressed in various tissues including the brain, heart, lung, kidney, skeletal muscle, and testis. The expression is reported to be up-regulated in endothelial cells and microglia by inflammatory signals. CX3CR1, a specific receptor for fractalkine, mediates both leukocyte migration and adhesion.
Synonyms	Neurotactin, FKN, NTT, SCYD1
Note	Centrifuge vial prior to opening!
Reference Data

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