FGF1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, FN, IHC, WB
Recommend Dilution	Immunohistochemistry: This antibody stained formalin-fixed, paraffin-embedded sections of human normal kidney. The recommended concentration is 0.050 µg/mL-0.125 µg/mL with an overnight incubation at 4˚C. An HRP-labeled polymer detection system was used with a DAB chromogen. Heat induced antigen retrieval with a pH 6.0 sodium citrate buffer is recommended Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hFGF-acidic (30 ng/ml), a concentration of 0.46 - 0.9 µg/ml of this antibody is required. ELISA: To detect hFGF-acidic by direct ELISA (using 100 µl/well antibody solution) a concentration of at least 0.5 µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hFGF-acidic. Western Blot: To detect hFGF-acidic by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hFGF-acidic is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived 16.8 kDa recombinant hFGF-acidic.
Specificity	Human Fibroblast Growth Factor acidic (hFGF-1)
Formulation	PBS, pH 7.2 without preservatives. State: Aff - Purified State: Lyophilized purified Ig fraction.
Reconstitution Method	Restore in sterile water to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity chromatography.
Conjugation	Unconjugated
Storage Condition	Store lyophilized at 2-8°C for 6 months or at -20°C long term. After reconstitution store the antibody undiluted at 2-8°C for one month or (in aliquots) at -20°C long term. Avoid repeated freezing and thawing.
Gene Name	fibroblast growth factor 1
Database Link	Entrez Gene 2246 Human UniProt ID P05230
Background	The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these two growth factors.
Synonyms	FGFA, Acidic fibroblast growth factor, ECGF-beta, HBGF-1, HBGF1
Note	Centrifuge vial prior to opening!
Reference Data

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