FGF1 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Human FGF-Acidic by direct ELISA (using 100 µl/well antibody solution), this antibody can be used at a concentration of ~1.0 µg/ml. This Biotin conjugated antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-acidic. Sandwich ELISA: To detect Human FGF-Acidic by Sandwich ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 µg/ml. This Biotin conjugated antibody in conjunction with Affinity purified antibody (PP1006P) as a Capture antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-acidic. Western Blot: To detect Human FGF-Acidic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-acidic is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.Coli derived recombinant Human FGF-acidic.
Specificity	Human FGF-acidic.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtred) purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	fibroblast growth factor 1
Database Link	Entrez Gene 2246 Human UniProt ID P05230
Background	The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these two growth factors.
Synonyms	FGFA, Acidic fibroblast growth factor, ECGF-beta, HBGF-1, HBGF1
Note	Centrifuge vial prior to opening!
Reference Data

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