Exodus 2 (CCL21) Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA Direct: To detect hExodus-2 by direct ELISA (using 100 ml/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 μg/ml. Used in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hExodus-2. Sandwich: To detect hExodus-2 by Sandwich ELISA (using 100 ml/well antibody solution) this antibody can be used at a concentration of 0.25-1.0 μg/ml. Used in conjunction with anti Human Exodus-2 PP1005P1 or PP1005P2 allows the detection of at least 0.2-0.4 ng/well of recombinant hExodus-2. Western Blot To detect hExodus-2 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hExodus-2 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Human
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) recombinant hExodus-2 (human Exodus-2).
Specificity	Specific for Exodus-2.
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography.
Conjugation	Biotin
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	C-C motif chemokine ligand 21
Database Link	Entrez Gene 6366 Human UniProt ID O00585
Background	Human CCL21 is a beta or C-C chemokine identified in the Expressed Sequence Tag (EST) database by three independent groups. CCL21 contains four conserved cysteine (C) residues which are characteristic of beta-chemokines. Human CCL21 cDNA encodes a 134 amino acid precursor protein, a 23 amino acid signal peptide and a 111 amino acid mature protein. Unlike other C-C chemokines, CCL21 is not chemotactic for monocytes and neutrophils. A growing body of work suggests that CCL21 influences lymphocyte homing to secondary lymphoid organs, integrin-mediated lymphocyte adhesion, and may act via the EBI1 ligand chemokine (ELC) receptor, CCR7.
Synonyms	Small-inducible cytokine A21, Beta chemokine Exodus-2, SCYA21, CCL-21, SLC
Note	Centrifuge vial prior to opening!
Reference Data

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