Ccl9 Rabbit Polyclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Direct ELISA: To detect Murine MIP-1 gamma by Direct ELISA (using 100 µl/well antibody solution) this antibody can be used at a concentration of ~1 µg/ml. This Biotinylated polyclonal antibody allows the detection of at least 0.2-0.4 ng/well of recombinant Murine MIP-1 gamma. Sandwich ELISA: To detect Murine MIP-1 gamma by Sandwich ELISA (using 100 µl/well) a concentration of 0.25-1.0 µg/ml of this antibody is required. This Biotinylated polyclonal antibody, in conjunction with anti-Murine MIP-1 gamma (PP024P1 or PP024P2) as a Capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Murine MIP-1 gamma . Western blot: To detect Murine MIP-1 gamma by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Murine MIP-1 gamma is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity	Mouse
Host	Rabbit
Clonality	Polyclonal
Immunogen	Highly pure (>98%) E.coli derived recombinant Murine MIP-1-gamma
Specificity	This antibody reacts with Murine MIP-1 gamma
Formulation	PBS, pH 7.2 without preservatives. Label: Biotin State: Lyophilized (sterile filtered) purified Ig fraction. Label: conjugated
Reconstitution Method	Restore in sterile PBS containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml.
Purification	Affinity Chromatography using an immobilized Murine MIP-1 gamma Matrix.
Conjugation	Biotin
Storage Condition	The lyophilized antibody is stable at Room Temperature for up to one month. The reconstituted antibody can be stored at 2-8°C for at least two weeks or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	chemokine (C-C motif) ligand 9
Database Link	Entrez Gene 20308 Mouse UniProt ID P51670
Background	This chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils may play a role in neoplasia and inflammatory host responses and can bind heparin. Its highest expression is found in the small intestine and peripheral blood cells. It is induced by interferon gamma, mitogens and interleukin 1 and belongs to the intercrine beta (chemokine CC) family.
Synonyms	C-C motif chemokine 9, CCL-9, MIP1-gamma, Mrp2, MRP-2, CCF18, Small-inducible cytokine A9, Scya10, Scya9
Note	Centrifuge vial prior to opening!
Reference Data

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.