Csf2 Rabbit Polyclonal Antibody

CAT#: PP003B2

Csf2 rabbit polyclonal antibody, Biotin

Conjugation: Unconjugated Biotin



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CNY 4,790.00


货期*
5周

规格
    • 50 ug

Product images

Specifications

Product Data
Applications ELISA, WB
Recommend Dilution ELISA:
(Direct): To detect mGM-CSF by direct ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mGM-CSF.
(Sandwich): To detect mGM-CSF by sandwich ELISA (using 100 μl/well antibody solution) a concentration of 0.25-1.0 μg/ml of this antibody is required. This biotinylated polyclonal antibody, in conjunction with Polyclonal Anti-Murine GM-CSF (PP003P1 or PP003P2) as a capture antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mGM-CSF.
Western Blot:
To detect mGM-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mGM-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
Reactivity Mouse
Host Rabbit
Clonality Polyclonal
Immunogen Highly pure (>98%) recombinant mGM-CSF (murine GM-CSF)
Specificity Detects Murine GM-CSF.
Formulation PBS, pH 7.2 without preservatives.
Label: Biotin
State: Lyophilized purified Ig fraction.
Label: conjugated
Reconstitution Method Restore in sterile PBS containing 0.1% BSA to a concentration
of 0.1-1.0 mg/ml.
Purification Affinity chromatography.
Conjugation Biotin
Storage Condition Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer.
Avoid repeated freezing and thawing.
Gene Name colony stimulating factor 2 (granulocyte-macrophage)
Background The cytokine GM-CSF stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes. Used in myeloid reconstitution following bone marrow transplant, bone marrow transplant engraftment failure or delay, mobilization and following transplantation of autologous peripheral blood progenitor cells, and following induction chemotherapy in older adults with acute myelogenous leukemia.
Synonyms CSF2, GMCSF, Sargramostim, Molgramostin
Note Centrifuge vial prior to opening!
Reference Data
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.
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