Interferon gamma (IFNG) Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	ELISA: In a sandwich ELISA (assuming 100µl/well), a concentration of 4.0-8.0 µg/ml of this antibody will detect at least 500 pg/ml of recombinant Human IFN-gamma when used with Biotin anti-Human IFN-gamma antibody (cat. PP1019B) as the detection antibody at a concentration of approximately 2.0-4.0 μg/ml. Western Blot: To detect Human IFN-gamma by Western Blot analysis this antibody can be used at a concentration of 0.20-0.40 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human IFN-gamma is 1.0-3.0 ng/lane, under non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Recombinant hIFN Gamma
Specificity	This antibody recognizes Human IFN gamma.
Formulation	PBS, pH 7.2 without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified Ig fraction
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Purification	Affinity Chromatography on Protein A
Conjugation	Unconjugated
Storage Condition	Store the antibody prior to reconstitution at -20°C. Following reconstitution the antibody can be stored at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	interferon, gamma
Database Link	Entrez Gene 3458 Human UniProt ID P01579
Background	Interferon gamma is mainly produced by T lymphocytes and NK cells. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses,including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak antiviral and antiproliferative activity, and poteniates the antiviral and anti tumor effects of IFN alpha / beta (type I interferon). It is upregulated by IL2, FGF basic, EGF and downregulated by vitamin D3 or DMN. Labile at pH 2.
Synonyms	IFN-gamma, gamma IFN
Reference Data

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