BAFF (TNFSF13B) Mouse Monoclonal Antibody

Specifications

Product Data
Applications	ELISA, WB
Recommend Dilution	Sandwich ELISA: In a Sandwich ELISA (assuming 100 µl/well), a concentration of 5.0-6.0 µg/ml of this antibody will detect at least 1000 pg/ml of recombinant Human BAFF when used in conjunction with Biotinylated antigen affinity purified anti-Human BAFF (Cat.-No PP1203B1 or PP1203B1) as the detection antibody at a concentration of at least 0.25-0.50 µg/ml. Western Blot: To detect Human BAFF by Western Blot analysis this antibody can be used at a concentration of 0.25-0.50 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human BAFF is 1.0-2.0 ng/lane, under reducong or non-reducing conditions.
Reactivity	Human
Host	Mouse
Clonality	Monoclonal
Immunogen	Highly pure (98%) E.coli derived Recombinant Human BAFF (Cat.-No PA180)
Specificity	This antibody recognizes Human BAFF.  Other species not tested.
Formulation	PBS without preservatives State: Azide Free State: Lyophilized (sterile filtered) purified IgG fraction
Reconstitution Method	Restore in sterile water to a concentration of 1.0 mg/ml.
Concentration	1.0 mg/ml (after reconstitution)
Purification	Affinity Chromatography Protein G
Conjugation	Unconjugated
Storage Condition	The lyophilized antibody is stable at RT for up to 1 month. Following reconstitution store at 2-8°C for one month or at -20°C for longer. Avoid repeated freezing and thawing.
Gene Name	tumor necrosis factor superfamily member 13b
Database Link	Entrez Gene 10673 Human UniProt ID Q9Y275
Background	Members of the TNF superfamily regulate immune responses and induce apoptosis. A novel member in the TNF family was recently identified by several groups and designated BAFF (for B cell Activating Factor belonging to the TNF Family), BLyS (for B Lymphocyte Stimulator), TALL1 (for TNF- and ApoL- related Leukocyte-expressed Ligand), and THANK (for TNF Homologue that Activate Apoptosis, NFkB and c-jun N-terminal Kinase). BAFF/BLyS was characterized as a B cell stimulator since it induced B cell proliferation and immunoglobulin secretion. Two receptors for BAFF were recently identified and designated TACI and BCMA. BAFF also signals through a third TNF receptor BAFFR/BR3. BAFF and its receptors are involved in the development of systemic lupus erythaematosus and other B cell associated autoimmune diseases. Like TNFa and TRAIL, THANK was shown to activate NF-kB and c-jun N terminal kinase (JNK) and to induce apoptosis. The human BAFF gene codes for a 285 amino acid type II transmembrane protein containing a 46 amino acid cytoplasmic domain, a 21 amino acid transmembrane domain, and a 218 amino acid extracellular domain.
Synonyms	TNFSF13B, BLYS, TALL1, TNFSF20, ZTNF4
Reference Data

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